Mixed media (gouache, paint, graphite, colored pencil, glue, paper) on bristol paper. The illustration entitled,“Renal Diagram” features a drawing of a diagram highlighting the "Renal-Adrenal Interaction for Regulating Sodium Potassium and Water Excretion." It appeared in The Heart Bulletin March-April 1962 issue on page 33. There are publication markings. The reverse side has publication markings.